FastQCFastQC Report
Mon 27 Feb 2023
SRR3128538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627776
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147240.9045470629865535No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG31490.19345413619564364No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG30160.18528347880789495No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC29560.18159746795627899No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACGGTGT19930.12243699378784303No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18340.11266906503106079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51300.078.732721
ATAGCGG3550.075.496351
GTACGGG3650.074.674852
AGGGATG24100.074.0978855
CGTAGGG4700.072.990152
CGGGTAT2000.072.840175
AAGGGAC16400.070.203934
GTAGGGA12150.070.007013
TAAGGGA14800.069.855443
TATAGGG12850.069.8504942
GAATAGG8700.069.7187961
GGTAAGG9850.069.694111
TAGGGCG2850.069.253814
GAGGGAT21700.069.082844
ACGGGTA1500.068.924034
TAGGGCA7850.068.8442154
TAGGGTC5400.068.749984
AGTAGGG25250.068.489772
AGGGCAT13850.067.860885
ATAGGGA14050.067.229363
Produced by FastQC (version 0.11.5)