FastQCFastQC Report
Mon 27 Feb 2023
SRR3128537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834475
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182110.9927090857057196No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG35170.19171697624661008No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG33280.18141430109431855No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC30650.16707777429509804No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20390.11114896632551548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59400.081.214911
ATAGCGG5250.076.200531
TAGGGCA6900.074.243734
AGGGATG28600.072.962435
ACGGGAT6000.072.0640954
TAAGGGA14850.071.209583
TAGCGGG11500.070.3103942
AAGGGAC17200.069.9514
ACGGGTA1550.069.739454
ATAGGGC10250.069.695073
GAATAGG10200.069.213281
AGGGACT25800.068.675845
TAGGGTC5900.068.506034
GTAGGGA13300.068.200583
AGTAGGG25100.067.799072
TAGAGGG30650.067.638982
ATAAGGG23100.067.563952
AGGGAAT24250.067.445025
TAGGGCG2450.067.1404654
ATAGGGA16550.067.018753
Produced by FastQC (version 0.11.5)