FastQCFastQC Report
Mon 27 Feb 2023
SRR3128536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684819
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219280.8167403463697179No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG27250.10149659995701758TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC26850.10000674160902467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80800.081.5391541
CGTAGGG6500.078.843522
ATAGGGC14550.073.644223
GTAGGGA19600.072.173413
TAGGGCA13650.070.236734
AGTAGGG42450.068.891482
CGAAGGG27200.067.068052
GTAGGGC12150.066.143423
TAAGGGA24800.066.13643
TAGGGAT16150.065.47524
TAGGGAC13300.065.371264
ATAAGGG34900.065.33852
GAATAGG15350.065.301141
TAGAGGG49800.064.766082
AAGAGGG92750.064.6829152
TATAGGG22650.064.557252
AGGGATG38200.064.343575
AGGGCAT24350.064.270555
GTAAGGG39300.064.1246642
AAGGGCA37900.064.108874
Produced by FastQC (version 0.11.5)