FastQCFastQC Report
Mon 27 Feb 2023
SRR3128535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518360
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205160.8146571578328753No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG30210.11995902095014216TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG29220.11602789116726758TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC29160.11578964087739639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72650.080.455681
CGTAGGG6300.073.105152
GTAGGGA19000.072.720383
ATAACGG2900.071.347031
AGTAGGG40600.068.757942
GAATAGG14100.067.701481
ATAGGGC15600.067.4816743
CCGTACA7750.067.310643
ACGGGAT6650.066.430674
CGTACAC7800.066.276654
TAGAGGG46450.065.7642752
AGGGATG32850.065.381065
AAGAGGG86900.065.221382
TAGGGCA12900.065.211734
ATAGGGA24350.064.655923
AATAGGG36150.064.61162
TAAGGGA21250.063.472473
TAGGGCG4750.063.321154
GTAGGGC12550.063.285683
TCCGTAC8400.063.2210352
Produced by FastQC (version 0.11.5)