SRR3128532.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1520659
Sequences flagged as poor quality	0
Sequence length	100
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13834	0.909737160007602	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	4489	0.29520096221440834	RNA PCR Primer, Index 10 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTTTGTC	4486	0.29500367932587124	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	4456	0.2930308504404998	RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	4063	0.26718679204213436	TruSeq Adapter, Index 10 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	2232	0.14677846907163278	No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	1888	0.12415669785270729	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1834	0.12060560585903873	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	165	0.0	85.4445	4
GGTCGAT	40	1.036824E-9	82.24304	6
CGTTTTT	4945	0.0	81.76716	1
GTAGGGC	695	0.0	75.05346	3
TAGGGCA	735	0.0	73.52877	4
ACTAGGG	730	0.0	72.09875	2
ATAGGGA	1045	0.0	71.9509	3
AGTAGGG	2515	0.0	71.75063	2
CGAAGGG	1485	0.0	71.201416	2
AGGGCAT	1360	0.0	70.84192	5
AGGGAAT	1970	0.0	70.61564	5
AGGGATG	2010	0.0	70.61326	5
CGGGTAT	160	0.0	70.49635	5
AGACGGG	1030	0.0	69.80503	2
TAAGGGA	1165	0.0	68.57337	3
CCGTACA	2170	0.0	68.4321	3
AGTAAGG	880	0.0	68.387085	1
TAAGCGG	365	0.0	68.26998	1
AGGGCAC	1120	0.0	67.97863	5
CGTACAC	2185	0.0	67.53439	4