SRR3128531.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128531.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1702587
Sequences flagged as poor quality	0
Sequence length	100
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17544	1.0304319250646221	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	5041	0.296078849421498	RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	4913	0.28856087823999593	RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	4293	0.25214570532959546	TruSeq Adapter, Index 10 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	2614	0.1535310677222368	No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTTTGTC	2360	0.1386125936589437	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2059	0.12093361455244284	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5825	83.44747	1
TAGGGCG	310	81.866234	4
ACGGGTA	160	79.30792	4
GTAGGGC	855	73.65656	3
AGGGATG	2255	72.31955	5
AGAGGGC	2470	71.7327	3
AGTAGGG	2560	70.8735	2
TAGGGCA	965	70.61768	4
AGGGCAT	1535	70.11321	5
ATAGGGC	1040	70.04403	3
AAGGGAC	1480	69.543274	4
AGGGAAT	2215	69.381996	5
TGTAGGG	1165	68.5898	2
ATAGGGA	1360	68.422516	3
TAGAGGG	2625	68.40228	2
AGGGTAC	560	67.97821	5
GGTAAGG	1070	67.72449	1
AGGGCAC	1305	67.704895	5
CGAAGGG	1635	67.55953	2
AGAAGGG	5500	67.5151	2