FastQCFastQC Report
Mon 27 Feb 2023
SRR3128530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365466
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181820.7686434723644305No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG28720.12141370875759787Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG26690.11283189020683451RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC24330.10285499770446922RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61650.077.936891
TAGGGCA13700.077.5265354
ATAGGGC11400.074.204483
CGTAGGG5850.073.941332
ATAACGG2300.073.6588751
ACGGGTA2850.070.90654
GTAGGGA19700.069.65933
GAGGGAT30500.069.3386154
ATAGGGA21350.068.458133
TAGGGAT17300.068.456854
ATAAGGG30100.068.260632
TAAGGGA20950.068.194923
AAGAGGG82350.068.0562442
AGGGATG31650.067.710135
AGGGCAT20500.067.170145
TAACGGG8400.067.1672062
GGGCGAT10350.066.7481846
AGGGAAT28050.066.185
CCGTACA8950.066.162213
ATAGAGG15600.065.763111
Produced by FastQC (version 0.11.5)