FastQCFastQC Report
Mon 27 Feb 2023
SRR3128529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233362
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165810.7424233062083084No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG32590.1459235000864168Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG30760.13772957541142009RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC28590.12801328221757152RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55750.080.731921
ATAGGGA21000.074.2928163
ATAGGGC9700.072.668733
TATAGGG16250.070.560962
AGGGATG31500.069.225265
ATAACGG2600.068.7366641
TAAGGGT9000.068.4001163
TAGGGAT15900.067.978014
CGTAGGG5900.067.700982
TAGGGCA10300.067.5246354
GTAGGGC9600.067.062143
CGAAGGG20550.067.001392
GTAGGGA17050.066.698953
GAGGGAT26650.066.654944
GTACGGG6500.066.512382
AAGAGGG72500.066.178322
TAAGGGA18400.066.146973
AGGGAAT25450.066.107765
ATAGAGG14300.066.10561
AAGGGGC55350.065.9691854
Produced by FastQC (version 0.11.5)