FastQCFastQC Report
Mon 27 Feb 2023
SRR3128528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500154
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203750.8149497990923759No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG36550.14619099463473048Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG34290.13715155146442978RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC30090.12035258628068511RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25340.10135375660859292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66550.077.986161
ATAGGGA25300.071.519793
ATAGGGC13100.071.3950043
TAGGGCA12400.070.87714
GTAGGGA20400.070.728463
GAGGGAT33550.069.202294
TAAGGGA21000.068.9314653
TAGGGCG3950.067.820944
AAGAGGG86800.067.640512
TAAGGGT9450.067.638373
AGGGATG34550.067.607425
CGAAGGG23450.066.952652
GATAGGG34050.066.817842
AGGGAAT32850.066.814015
GTAGGGC10500.066.693433
AGTAGGG39200.066.5535352
ATAGAGG15300.066.4281851
ATAAGGG32150.066.380292
CGTAGGG5250.066.2577442
AGGGATA26700.066.009445
Produced by FastQC (version 0.11.5)