FastQCFastQC Report
Mon 27 Feb 2023
SRR3128527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338541
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187300.8009267316673088No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43200.18473056491205414No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG25480.10895682393423933No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC24410.10438132151627873No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG23570.10078933830965546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64300.079.120211
AGGGATG50350.076.445725
ATAGGGA24900.073.420783
GTAGGGC12800.072.698243
CGAAGGG22100.072.1256562
CGTAGGG7700.071.445922
GAGGGAT37100.070.558614
AGTAGGG35900.069.809592
AGGGCAT22250.069.7031865
AAGGGAT55700.069.5249564
CGAGGGA14850.068.9920043
ACGGGTA3100.068.221234
AGTACGG2350.068.0900651
AGGGAAT31450.067.693395
AGAGGGC34500.067.294033
TAAGGGA21550.067.16953
AGGGATA32250.066.8885355
TAGGGCA12950.066.775444
GGATGGC36050.066.486527
TAGAGGG41000.066.401372
Produced by FastQC (version 0.11.5)