FastQCFastQC Report
Mon 27 Feb 2023
SRR3128526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227632
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175860.789448167381327No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41060.1843212882558699No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG28160.12641226198941297No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC27560.1237188189072522No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG25850.11604250612309394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62450.080.9988251
CGTAGGG7700.076.287342
TACGGGT2450.074.805183
AGGGATG46550.073.597065
AGTAAGG12550.072.29571
AGTAGGG35600.072.073532
GTAGGGC9800.071.928063
TAGGGCA13350.071.8095254
ATAGGGA24250.071.119293
AGGGTAT12300.070.68375
AGGGCAT22350.070.650375
TAAGGGA17450.070.556823
AGGGAAT28050.070.534555
GTAGGGA21300.070.379193
GAGGGAT34800.069.949354
CGAAGGG22850.069.9239352
AAGGGAT49900.068.558924
TAGGGTA8600.068.303774
ACGGGTA2700.067.878784
AGGGATA28200.067.826275
Produced by FastQC (version 0.11.5)