FastQCFastQC Report
Mon 27 Feb 2023
SRR3128525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534440
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215310.8495367813008001No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49010.19337605151433845No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG32150.12685248023231954No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG28680.11316109278578307No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC28640.11300326699389214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72250.080.6109541
AGGGATG54550.075.128445
GAGGGAT41850.074.2315754
CGTAGGG8000.072.8575062
CGAAGGG25550.071.565112
AGTAAGG17600.070.2998351
TAAGGGA23400.070.296683
ATAGGGA30600.070.190353
AGGGCAT25750.069.904345
GTAGGGC12150.069.240373
ACGGGTA2600.068.68994
AAGGGAT58600.068.492484
TAGGGCA16300.068.335024
AGGGAAT33500.068.3229755
AGGGTAC9850.068.231125
AGGGATA35700.068.062045
AGTAGGG43600.067.704222
AAGAGGG84050.066.886132
TAGAGGG46050.066.858142
CGAGGGA17200.066.672093
Produced by FastQC (version 0.11.5)