FastQCFastQC Report
Mon 27 Feb 2023
SRR3128524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284525
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186530.8164935818167891No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37680.1649358181678905No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG34630.1515851216336No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC33760.14777689016316303No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG32030.14020419999781136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65500.082.6171341
ATAGCGG4700.076.090311
CGTAGGG6750.075.921592
ATAGGGC14050.073.255333
AGGGATG33550.072.842075
GAATAGG14600.071.872971
TAGGGCA12900.071.04224
ACGGGAT8250.070.638134
CGAAGGG20950.069.569722
TAAGGGA21850.069.043863
TAGCGGG12850.068.785562
AGGGAAT29700.068.359485
TGTAGGG15000.068.329442
TAGAGGG41000.068.115382
AGTAGGG33500.068.067462
GAGGGAT29950.067.7888644
AAGGGTA18150.067.582674
AGATAGG12700.067.063841
AAGGGAT38850.067.017814
AGGGCAT22100.066.986885
Produced by FastQC (version 0.11.5)