FastQCFastQC Report
Mon 27 Feb 2023
SRR3128523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191836
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172970.7891557580037922No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG40880.18651030460308163No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC38860.17729428661633445No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG36950.1685801309952022No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35240.16077845240246078No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGGACTGG24310.11091158280090299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57250.083.066611
ATAGGGC12650.071.701963
TAAGGGA20850.071.67793
ATAGCGG4950.071.270021
AGTAGGG33700.070.703722
AGGGTAC7550.070.338935
AGGGCAT21300.070.163585
AGGGATG31450.069.9341665
AGAGGGC31050.069.624243
AGGGAAT28200.069.494615
CGTAGGG5150.069.353852
TAGGGCA10900.068.5558854
TAGGGCG4400.068.35994
TAGCGGG11800.068.10492
AAGGGAT36750.067.394964
CGAAGGG20950.067.29792
AAGGGCA36650.066.809454
CTACGAC8200.066.482665
ACGGGAT7150.066.3879854
TATAGGG14600.066.309942
Produced by FastQC (version 0.11.5)