FastQCFastQC Report
Mon 27 Feb 2023
SRR3128522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449824
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212190.866143853599279No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG45770.1868297477696357No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41780.17054286348733624No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC40140.16384850503546378No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG39340.1605829643272333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71450.083.5048751
TAGGGCA11800.076.073224
ATAGGGC14250.074.537353
AGGGATG39250.073.640355
CGTAGGG7050.073.339362
TAAGGGA26000.073.027833
ATAGCGG5850.072.390481
GAGGGAT33650.071.2304154
GTAGGGC10500.071.168573
AGTAGGG38500.070.933112
TAAGCGG5000.070.580721
GAATAGG16400.070.293811
TAGAGGG40950.070.247542
AGGGAAT31650.069.4948355
CGAAGGG21350.069.3499762
TGTAGGG18150.068.369262
AAGGGTA17050.068.360894
TATAGGG16850.067.7859952
ATAGGGA23650.067.764723
GTAGGGA18500.067.575663
Produced by FastQC (version 0.11.5)