FastQCFastQC Report
Mon 27 Feb 2023
SRR3128521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891494
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169290.8950068041452948No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG41990.22199383133121225Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG39000.20618622105066153RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC36680.19392078431123758TruSeq Adapter, Index 10 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGATTACC24810.13116615754530545No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19590.10356892488160153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55750.081.130261
ATAGGGC10600.076.7030643
CGAAGGG20300.073.8918842
GGTAAGG10650.072.918661
GGGTACG2250.071.0180746
ATAGCGG5150.070.370051
TAGGGTA5250.069.8244864
TATAGGG14300.069.382272
ATAGGGA16950.069.040213
AAGGGTA15200.068.949914
CGTAGGG5050.068.903692
ACGGGAT5400.068.755234
AGGGATG24150.068.695785
AGTAGGG29500.068.540712
TAGCGGG11750.068.432182
AGGGCAT16650.068.308325
CGAGGGA10400.068.23643
TAGGGCA9400.067.996024
AGGGTAC9000.067.884925
ATGCGGG9550.066.9634252
Produced by FastQC (version 0.11.5)