Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128520.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1810217 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 15510 | 0.8568033556197958 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 4796 | 0.2649406120923624 | Illumina PCR Primer Index 2 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 4398 | 0.24295429774441407 | RNA PCR Primer, Index 10 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC | 4232 | 0.23378412643346075 | TruSeq Adapter, Index 10 (95% over 23bp) |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGATTACC | 3313 | 0.18301673224812273 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 1866 | 0.10308156425445127 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 1830 | 0.10109285240388306 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5120 | 0.0 | 80.72497 | 1 |
ATAGCGG | 375 | 0.0 | 78.99475 | 1 |
AGTAGGG | 2595 | 0.0 | 76.06291 | 2 |
GGTAAGG | 955 | 0.0 | 72.86975 | 1 |
ATAGGGC | 1015 | 0.0 | 72.69334 | 3 |
CGTAGGG | 570 | 0.0 | 72.555244 | 2 |
AAGGGTA | 1380 | 0.0 | 72.537315 | 4 |
AGGGTAC | 820 | 0.0 | 71.64026 | 5 |
ATAGGGA | 1765 | 0.0 | 70.82684 | 3 |
AGATAGG | 910 | 0.0 | 69.75596 | 1 |
TATAGGG | 1515 | 0.0 | 69.175644 | 2 |
CCGTACA | 1460 | 0.0 | 68.88456 | 3 |
ACTAGGG | 835 | 0.0 | 68.664825 | 2 |
GTACGGG | 445 | 0.0 | 68.64586 | 2 |
AGGGAAT | 2120 | 0.0 | 67.83386 | 5 |
TCCGTAC | 1555 | 0.0 | 67.3962 | 2 |
CGAGGGA | 855 | 0.0 | 67.05863 | 3 |
ATCCGTA | 1570 | 0.0 | 66.78734 | 1 |
AGGGATG | 2015 | 0.0 | 66.47078 | 5 |
TGTAGGG | 1075 | 0.0 | 66.45017 | 2 |