FastQCFastQC Report
Mon 27 Feb 2023
SRR3128520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810217
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155100.8568033556197958No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG47960.2649406120923624Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG43980.24295429774441407RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC42320.23378412643346075TruSeq Adapter, Index 10 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGATTACC33130.18301673224812273No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18660.10308156425445127No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18300.10109285240388306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51200.080.724971
ATAGCGG3750.078.994751
AGTAGGG25950.076.062912
GGTAAGG9550.072.869751
ATAGGGC10150.072.693343
CGTAGGG5700.072.5552442
AAGGGTA13800.072.5373154
AGGGTAC8200.071.640265
ATAGGGA17650.070.826843
AGATAGG9100.069.755961
TATAGGG15150.069.1756442
CCGTACA14600.068.884563
ACTAGGG8350.068.6648252
GTACGGG4450.068.645862
AGGGAAT21200.067.833865
TCCGTAC15550.067.39622
CGAGGGA8550.067.058633
ATCCGTA15700.066.787341
AGGGATG20150.066.470785
TGTAGGG10750.066.450172