FastQCFastQC Report
Mon 27 Feb 2023
SRR3128519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010039
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199820.9941100645310862No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG51970.25855219724592404Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG49640.24696038236074028RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC47780.23770683056398406TruSeq Adapter, Index 10 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21670.10780885346005724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60050.080.5514761
ACGGGAT5150.075.7453844
ATAGGGC11050.075.708223
AAGGGTA16200.074.559584
TAGGGCA9400.071.9979254
AGGGTAC10150.071.771335
GGTAAGG11150.071.7410051
AGTAGGG27350.071.323082
CGTAGGG5850.070.707612
AGGGAAT23100.070.396235
TAAGGGT8700.069.147433
ATAAGGG25600.068.670622
AGGGCTC16050.067.350085
AGGGATG24950.067.060195
TAGAGGG32250.066.462162
AATGCGG4850.065.972131
TAGGGTA6350.065.8721164
GTACGGG4500.065.806282
GAGGGAT21550.065.645444
ATAGGGA17050.065.605153
Produced by FastQC (version 0.11.5)