FastQCFastQC Report
Mon 27 Feb 2023
SRR3128517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934931
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528372.7306916887475574No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT55480.2867285706828822No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT53860.2783561791092292No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT53370.27582378906534655No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG45300.23411687548548243No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC25990.13432003518471716No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC25740.13302799944804233No Hit
CGTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC25510.13183932657030148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168000.088.3931351
GTAGGGC7550.075.9399643
ATAGCGG4250.071.918431
ATAGGGC11000.071.7751543
AGGGTAC7050.067.9989555
AGAGGGC28000.067.807983
AGTAGGG27650.067.476552
AGGGCTC18850.067.3199165
TAGGGCA9650.066.720914
CGTAGGG5300.066.503262
TATAGGG14350.066.481632
ATGAGGG27950.066.079732
AGGGATG27850.065.984635
AAGGGCA26250.065.168974
GTAGGGT6150.064.9533163
TAGAGGG31400.064.5067442
CCGTACA3500.064.451153
GGTAAGG10450.064.348061
AGGGCAT15100.064.118225
AAGAGGG62200.063.995662