FastQCFastQC Report
Mon 27 Feb 2023
SRR3128515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786829
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333381.1962700258968169No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36580.1312602962004486No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG28280.10147734216918226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114250.083.962271
ATAGCGG5400.075.833611
AGGGATG44250.070.4151155
ATAGGGC16450.069.99483
CGTAGGG9100.069.2458952
AGTAGGG45850.067.6916662
GAATAGG17650.067.470211
TAGGGTA8300.067.380544
ATAGGGA25800.067.2159353
ATAGAGG20900.066.4372941
TATAGGG21050.066.349092
TAGGGCA14450.066.3480154
GTAGGGC12900.065.212213
AGATAGG15100.065.1486741
TAGAGGG50550.065.1189652
ACGGGTA4050.064.9828344
AATAGGG41500.064.588822
CCGTACA7850.064.058943
GGTAAGG18150.064.055521
TAGGGAT19500.063.867054
Produced by FastQC (version 0.11.5)