FastQCFastQC Report
Mon 27 Feb 2023
SRR3128514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626039
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311391.1857782767125697No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35780.1362508325276205No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG32070.12212309108889853No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT28390.10810959014698562No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC28210.10742414716613119TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105650.083.7690661
CGTAGGG8250.073.48742
TAGGGTA10100.072.589214
AGGGATG38200.070.741355
ATAGGGC14200.069.5037163
AGTAGGG44550.068.571362
TAGAGGG47700.068.181222
TAGGGCA11600.068.064414
ATAGCGG5200.067.825261
AGGGAAT28550.066.668055
ATAGAGG19050.066.650331
AGATAGG14400.066.292911
GGTAAGG16000.066.129631
CCGTACA8500.065.796853
GTAGGGC13250.065.26483
GATAGGG37500.064.919562
CGAAGGG26450.064.677442
AAGGGGC72550.064.325134
GAGGGAT34100.064.224444
ATAGGGA24300.064.017523
Produced by FastQC (version 0.11.5)