FastQCFastQC Report
Mon 27 Feb 2023
SRR3128513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956945
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380811.2878494527290836No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40680.1375744222499911No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG36090.12205164451824432No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT31180.10544666877469822No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC30450.10297790456028097TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129000.083.6006851
ATAGGGC18750.073.191313
TAGGGCG4450.070.76084
AGATAGG16550.070.508041
ATAGCGG5550.070.3671651
TAGGGCA12150.068.8528064
AGGGATG46850.068.415225
GTAGGGC13250.067.747833
TAGAGGG55100.067.7361152
ATAGAGG20750.066.894261
AGTAGGG49650.066.177342
ATAGGGA27850.066.151373
GGTAAGG17100.066.038931
TAGGGTA7500.065.170344
GTACGGG9700.064.9358442
GATAGGG40750.064.827622
CGAAGGG31000.064.443422
ATGCGGG17700.064.267812
GAATAGG20200.064.056951
AAGGGGC79450.064.0046164
Produced by FastQC (version 0.11.5)