FastQCFastQC Report
Mon 27 Feb 2023
SRR3128512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867947
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78910.9091568955247268No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGGTACA58530.6743499315050343No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG44210.5093628988866832No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC37680.43412789029744897No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG31020.35739509440092543No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26860.3094659005676614No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19870.2289310291987875No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATG19540.2251289537264372No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT13320.15346559179304728No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTGGTACATC12400.14286586623376774No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG12140.13987029161918874No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT11520.13272699830750034No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT11130.12823363638563184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38550.087.7617951
CGCTACG5350.086.962423
GTTACGG650.086.869811
CTACGAC5550.082.981885
ACCGGGA800.082.2409743
AGGGTAT4750.082.11735
CGAAGGG6550.081.820832
TAAGCGG2150.080.977481
AGGGATG20800.079.9816065
CGAGGGA6000.079.107993
GCTACGA5850.078.726414
TAGCGGG2400.078.3518142
GATGGCA11200.078.0450068
GAGGGAT19600.077.685354
TACGACC5950.077.4032756
AACGAGG2250.077.378481
GTAGGGC4500.077.28043
GGCGATC551.8189894E-1276.900667
AGGGATT17550.076.851975
GAGGGTA3550.076.7803044
Produced by FastQC (version 0.11.5)