FastQCFastQC Report
Mon 27 Feb 2023
SRR3128511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850133
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71230.8378688981606408No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGGTACA70670.8312816935703002No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG51040.6003766469481834No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC42450.499333633678495No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG37660.4429895087004034No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATG25700.3023056392352726No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24790.29160143177596914No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23680.278544651248687No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTGGTACATC16050.1887939887052967No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG15070.17726638067220069No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT14640.17220834857604633No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT13060.153623021339014No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT12810.15068230500404053No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT9970.11727576743874193No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC9670.11374690783677377No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTTGGTACATCGT9420.11080619150180031No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT9390.11045330554160349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT700.087.284135
CGTTTTT36700.087.123891
TTGACGG550.085.493231
AGGGATG18200.082.6358645
CGAGGGA6400.082.2436753
GCTACGA5200.082.2436754
TAAGCGG1950.081.985811
TAGGGCA4550.081.5981144
ACGGGAT4450.081.319584
GAATAGG4300.080.9203341
CTACGAC5350.079.942485
CGCTACG5350.079.9377753
CGAAGGG8300.079.837232
GGAGCTA25800.078.145159
AGGGATC11200.078.0521555
GGGCGAT2350.077.9939966
ATAGCGG1450.077.828321
CGGGAAT3150.077.58595
ACGGGAC1950.077.122274
GTAAAGG5000.077.114881
Produced by FastQC (version 0.11.5)