FastQCFastQC Report
Mon 27 Feb 2023
SRR3128510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927599
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93541.0084098840123803No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG55940.6030623146424263No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC48590.5238254892469698No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG40430.43585644227732023No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGGTACA38560.4156968690134422No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28120.30314823539050817No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT15540.16752928797896505No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14590.15728779354009653No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT14500.1563175466985195No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG14150.15254436453683112No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT13510.14564483144117232No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATG12500.13475650577458578No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT10240.11039252953054068No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT9830.10597251614113427No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT9380.10112128193324917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44450.087.22161
CGCTACG5500.086.304883
CGTAGGG2450.084.435912
CGAAGGG7800.083.181182
GGGCGAT3300.082.602036
TCCGCTA5850.082.03761
ACGGGAT5850.081.141354
GGAGCTA23950.080.455379
AGGGAAT20000.080.366085
AGGGATG21350.079.02665
CTACGAC5950.078.987755
GCTACGA5950.078.987754
AAGGGAT26050.078.119034
TAAGCGG2000.077.63411
CGAGGGA6550.077.49243
GAGGGAT18400.077.392974
CGGGATG4500.077.2851265
TAGGGCA5650.076.527244
ATAGGGA13700.076.499923
GCATAGG2650.076.346861
Produced by FastQC (version 0.11.5)