FastQCFastQC Report
Mon 27 Feb 2023
SRR3128509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742421
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154860.8887633930031835No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG24040.13796895239439835TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG23670.13584547018200538RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC20280.11638978180359397RNA PCR Primer, Index 34 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19050.10933063823266594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51750.082.389351
ATAACGG1450.074.6471251
ATAGGGC9400.071.9928363
ACGGGAT6150.071.06614
AGTAGGG29550.069.857172
CGAAGGG19950.069.767432
ATAGGGA15950.069.240763
TAGGGCA10200.069.110774
CGTAGGG5650.069.077032
TAGGGAT13350.068.996884
AGGGATG24350.068.514755
GTAGGGA16500.068.3568343
TAACGGG6550.068.2002942
TAGCGGG8950.067.249782
AAGGGAT29850.066.911274
TATAGGG14200.066.5597462
AGGGAAT20550.066.3194355
ATGAGGG26550.066.238582
AAGGGCA27300.065.4147344
AAGGGGC43100.065.313684
Produced by FastQC (version 0.11.5)