FastQCFastQC Report
Mon 27 Feb 2023
SRR3128508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663529
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146870.8828821138675671No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG28410.17078151327689509TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG26710.16056227453804534RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC22970.13807994931257586RNA PCR Primer, Index 34 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16810.1010502371765085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50350.081.245091
ACGGGAT4900.073.8543554
ATAGGGC7300.072.106873
ATAGGGT7450.071.28593
CGAAGGG16650.071.1324542
ATAGGGA15800.070.4973453
TAGGGTA4350.068.06644
TAGCGGG9750.067.966672
GAATAGG9450.067.170611
GCATAGG3500.067.170611
CGTAGGG5950.067.140332
TAGGGCA7750.066.707154
TAGGGTC4400.066.224774
AAGAGGG58300.065.7007752
AGGGAAT18450.065.466375
AGAGGGC24450.065.355413
AGGGATG24250.065.3135
AGTAGGG26500.065.265462
ATGAGGG24050.065.074462
GATAGGG23050.065.04312
Produced by FastQC (version 0.11.5)