FastQCFastQC Report
Mon 27 Feb 2023
SRR3128507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878681
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180510.9608336912972453No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG30600.16288023352554265TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG28910.153884560497498RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC23510.1251409898753434RNA PCR Primer, Index 34 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19270.1025719640535035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57800.083.096581
ATAGCGG3750.072.75821
CGTAGGG6100.072.4329762
CGAAGGG19050.071.308482
TAGCGGG9900.071.218752
ATAGGGC10400.070.949093
AAGGGCG10700.068.959864
AGGGAAT20500.067.172935
TAGGGTC4550.067.1401754
TAGAGGG33700.066.949852
AGAGGGC25950.066.4674763
ATGAGGG30900.066.3233262
ATAGAGG11900.065.621541
AAGAGGG63850.065.592642
TAGGGCA10300.065.249824
GAGGGAT24700.064.884054
ATAGGGA17400.064.8253
ATAAGGG25500.064.331482
AGAGGGA46600.064.244223
AATAGGG24750.064.191832
Produced by FastQC (version 0.11.5)