FastQCFastQC Report
Mon 27 Feb 2023
SRR3128506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155871
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125951.0896544683619538No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC68470.5923671413159427No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG34680.3000334812448794No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGGGTGA32660.28255748262565633No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG31250.27035888953006No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC30150.2608422566186019No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15690.13574179125525254No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15020.12994529666372803No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATG14560.12596561380984556No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG13140.11368050586959963No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT12410.10736492221017743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44250.086.544871
TAGGGCA7150.084.789874
CGCTACG12000.080.676353
GCTACGA12100.080.3984
AGGGATG29050.079.59385
AGGGCGA4550.079.531585
ACGACCA12150.078.519967
AGGGCAT10900.078.4702765
GGTAAGG6550.078.291641
AGGGTAC5350.078.180155
AAGGGCG5350.078.180154
GAGGGAT19800.077.851854
CGTAGGG3100.077.329272
AGGGAAT21850.077.0002065
CGGGAAT3850.076.9024355
GGATGGC20950.075.597287
GAATGCC14800.075.2578
TCCGCTA12950.074.838941
GTAGGGC5350.074.6664353
GGAATGC16000.074.606057