FastQCFastQC Report
Mon 27 Feb 2023
SRR3128505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140159
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119691.0497658659888665No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66060.5793928741517631No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGGGTGA43360.38029783565274666No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG39790.34898641329849606No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG36610.32109556649555016No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC34040.2985548506831065No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATG19760.17330916126610413No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19200.16839756560269226No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15530.13620907259426096No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT13910.12200052799653381No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG13530.11866765951064719No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT11990.10516077143626458No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT11590.10165248881954185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41400.085.39191
CGCTACG12150.083.53843
CGGGAAT3950.082.091615
GGTAAGG6150.081.027031
AGGGAAT20100.079.7269445
TCCGCTA12900.079.444891
CTACGAC12800.079.303185
GCTACGA12900.078.6815264
TAGGGCA6900.078.317254
TAGCGGG4500.077.273022
AGGGATG28450.077.140325
AAGGGCA18000.076.4898454
GGATGGC18600.076.299517
ACGACCA13150.076.116997
CGTAGGG3150.076.079622
CGACCAA13150.075.759638
AGTAAGG5650.075.716781
AGGGCAT10650.075.455925
GGAATGC15950.075.423557
CGGGTAT507.094059E-1175.191165
Produced by FastQC (version 0.11.5)