FastQCFastQC Report
Mon 27 Feb 2023
SRR3128503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624184
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209110.7968572325721062No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55050.2097794971694058No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG33090.12609634080537035No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG31580.12034217112824405No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC28670.10925300969749073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75000.082.777041
TAGGGCG4600.074.5811164
AGGGATG55450.074.160125
CGTAGGG6950.073.072022
AGAGGGC39950.071.523823
AAGGGAT56050.071.1862264
AGGGATC27000.070.4944845
CGAAGGG24200.069.757562
AGGGCGA13150.069.690365
AGGGCAT24400.069.531455
GAGGGAT49400.069.3528754
AGGGAAT35500.069.236845
CGAGGGA17100.068.982913
TATAGGG21800.068.809182
ATAGGGC17450.068.6765753
ATAGGGA27700.068.543373
TACGGGT2400.068.536313
TAGGGCA16550.068.435734
CGGGATG12500.068.0506745
ATAGCGG3750.067.7780151
Produced by FastQC (version 0.11.5)