FastQCFastQC Report
Mon 27 Feb 2023
SRR3128502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493574
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191990.769939051337558No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52310.20977921649808667No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG37290.1495443888972214No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG35690.14312789594373376No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC32860.13177872403225252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70900.081.313611
CGTAGGG6500.075.91912
AGGGATG52100.074.2368855
AAGGGAT52100.074.057974
ATAGGGC15300.071.878583
TAAGGGA23400.071.701363
TAGGGCA14200.071.487784
AGAGGGC35300.070.829143
AGGGATC24600.070.302445
TACGGGT1750.069.8249053
GAGGGAT44050.069.7747344
GTAGGGC10900.069.418373
CGAAGGG25900.069.3168262
TAGAGGG42250.069.189262
TATAGGG18500.069.0990752
AGTAGGG40000.068.968892
CGAGGGA16150.068.968523
ATAAGGG33600.068.677952
AAGAGGG85250.067.477982
ACGGGTA2800.067.1379854
Produced by FastQC (version 0.11.5)