FastQCFastQC Report
Mon 27 Feb 2023
SRR3128501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799633
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241930.8641489795269593No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60130.2147781512791141No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG42250.15091263747784084No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG40880.14601913893713925No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC38010.1357677952788812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86650.082.8524551
TAGGGCG5200.077.727054
TAGGGCA17300.073.892414
CGTAGGG6950.072.367882
AGGGATG60950.072.173745
AGGGCAT26650.071.422475
AAGGGAT63350.070.92324
GTAGGGC14850.070.892233
TAAGGGA29600.070.3378453
ATAGGGC16450.070.282363
TACGGGT1750.069.825233
AATGCGG4650.069.809661
AGGGATC29100.069.769855
AGTAGGG48950.069.6197052
AGAGGGC40950.069.0907063
TAGAGGG49250.068.90932
AGGGATT41250.068.81615
TATAGGG23500.068.807772
AGGGAAT37750.068.722545
ATAGCGG5000.068.686641
Produced by FastQC (version 0.11.5)