FastQCFastQC Report
Mon 27 Feb 2023
SRR3128500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449671
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231510.9450656843306713No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG55050.22472405478123392No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG50130.2046397250896141No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC46510.1898622304791133TruSeq Adapter, Index 14 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31220.12744568556348995No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC27530.11238243829477511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71750.083.501631
ACGGGTA2550.082.9336854
ATAGGGC15200.072.9670943
TAGGGTA7950.072.710414
CGTAGGG5900.071.723572
AGGGAAT26250.071.612585
GTAGGGA15550.071.324753
AGTAGGG33950.070.909042
TAGGGCA13950.070.74634
GTAGGGC9850.070.1357963
ATAGCGG4500.070.0730061
TGTAGGG17050.070.045582
AGGGCAT21650.069.679625
GGTAAGG12600.069.1018451
CTACGAC6600.069.069525
GGGCGAT9950.068.4862756
AGGGATG29650.067.997245
AAGGGTA16700.067.820224
AGTAAGG15700.066.249271
CGAAGGG22100.065.953962
Produced by FastQC (version 0.11.5)