FastQCFastQC Report
Mon 27 Feb 2023
SRR3128499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298166
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210350.9152950657176201No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG60620.2637755497209514No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG55090.23971288409975608No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC53960.23479591987698015TruSeq Adapter, Index 14 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC33310.14494166217757987No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28920.12583947373688412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64450.082.383591
ACGGGTA2800.078.887284
TAGGGTA7550.075.941664
CGTAGGG5200.073.207972
AGGGAAT24350.072.376825
AGTAGGG34250.071.628622
ATAGGGC13000.070.496563
AGTAAGG14350.069.806741
GAATAGG11900.069.556131
TAAGGGA17450.069.2172553
GTAGGGT8450.068.967053
TCCGTAC18350.068.895812
CGTACAC17750.068.840224
AGGGCAT16500.068.643645
GTAGGGA15550.068.6075743
ATCCGTA18800.068.042571
CCGTACA18050.067.95793
ACTAGGG10050.067.342
TACGACC6850.067.2346656
AGATAGG12350.066.6408841
Produced by FastQC (version 0.11.5)