FastQCFastQC Report
Mon 27 Feb 2023
SRR3128498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570995
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262771.0220556632743354No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG66950.260405018290584No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG64160.2495531885515141No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC63620.24745283440846833TruSeq Adapter, Index 14 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34040.1324001019060714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80350.082.256781
CGTAGGG5550.078.764992
ATAGGGC16350.074.44883
ACGGGTA2550.073.7217254
GAATAGG13600.071.599951
AGGGCAT21400.070.935625
TAGGGTA8500.070.772864
TAGCGGG13100.069.969152
AGTAGGG38250.069.186322
TAGGGCA15950.068.9494554
AGGGAAT27000.068.929815
CTACGAC7850.068.849985
ATAAGGG32850.068.6829762
TACGACC7900.068.414226
GGTAAGG14050.068.302271
TAGAGGG39850.067.941862
AATAGGG32150.067.839122
ATAGGGT10300.067.074243
GTAGGGA17800.067.063993
ATAGGGA21600.066.797513
Produced by FastQC (version 0.11.5)