FastQCFastQC Report
Mon 27 Feb 2023
SRR3128497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584881
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63641.0880845847274916No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCAC62741.072696839186091No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG41150.7035619211429334No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC34510.590034554037488TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG26640.4554772680254616No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24190.4135884051627596No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG21490.3674251685385574No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17550.3000610380573142No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCATGCACTC14790.252871951730352No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT13970.23885200579263133No Hit
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG11530.19713411788038931No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG10360.1771300486765684No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9610.1643069273920678No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT8610.14720943234606695No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT8330.14242213373318677No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC8100.13848970987260656No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8060.13780581007076653No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCACTCG8050.13763483512030653No Hit
GAACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT7070.12087928997522573No Hit
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT6410.10959494324486518No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT6250.10685934403750508No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG5940.10155912057324482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33200.089.961921
CTAGCGG550.085.518561
ACGGGTA1100.085.452764
CGAGGGA4050.084.714283
CGGGTAG500.084.5982365
CGTAGGG900.083.589542
TAGCGGG2150.083.103552
CGCTACG3250.082.429053
AGGGAAT11250.081.464965
ATAGCGG700.080.631781
GTAGCGG1050.080.631781
CTAACGG353.841342E-880.631781
AGGGATG10750.080.444835
GCTACGA3350.079.968484
GGATGGC8150.078.427817
CGGGTAT1200.078.3316965
GGTACGT301.4554244E-678.3316967
TAGGGTC2050.077.9495854
GATGGCA7150.076.250158
GGTAAGG1800.075.7789461
Produced by FastQC (version 0.11.5)