FastQCFastQC Report
Mon 27 Feb 2023
SRR3128496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575687
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCAC83381.4483564853818134No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58341.013397905459043No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG45110.7835855247730104No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC38280.6649446661119671TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG31280.5433508138971351No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG29710.5160790499003799No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28820.5006192601187798No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCATGCACTC18250.3170125432743835No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16800.29182524531559684No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT15130.2628164262872012No Hit
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG13760.23901877235372693No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT11280.19593980756904358No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC10850.18847047093298963No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG10120.17578996920201428No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9460.16432540599318726No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT9080.15772459687295354No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCACTCG8730.15164490426221194No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8180.1420911015881894No Hit
GAACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT7850.1363588199837759No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT7160.12437314026545676No Hit
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT7100.12333090724647247No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG7040.12228867422748821No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT7010.12176755771799606No Hit
ATCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC6180.10735000095538026No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT6030.10474441840791959No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT5790.10057548633198249No Hit
GACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC5780.10040178082881843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG156.8883644E-494.041461
GGTACGT353.583409E-1094.00067
CCGTGTA156.900271E-494.00068
GTACGTG156.900271E-494.00068
CGTTTTT29500.089.1002961
GTAGGGT1400.087.271123
CGCTACG3300.085.4402543
ACGGGAT2600.084.947334
GCTACGA3350.084.165024
AGGGTAC2600.083.154395
CTACGAC3450.081.7396555
TAGCAGG3500.080.606971
ATTACGG353.8493454E-880.6069641
TATCGGG353.8657163E-880.557952
TAAGCGG1350.080.109391
AGGGATG9950.079.829665
TCGAAGG650.079.573541
TCCGCTA3550.079.471651
TACGACC3550.079.437136
AGGGAAT11200.079.313015
Produced by FastQC (version 0.11.5)