FastQCFastQC Report
Mon 27 Feb 2023
SRR3128495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624069
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73131.1718255513412779No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG53400.8556746129033809No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCAC43840.702486423776858No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC43110.6907889992933474TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG35670.5715714127764718No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20530.32897003376229234No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17660.28298152928602444No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT16050.2571830999456791No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG13920.2230522586444768No Hit
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG10910.1748204124864398No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG10670.17097468388912124No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCATGCACTC9980.15991821417183036No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT9910.15879654333094578No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9570.1533484278180778No Hit
GAACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT9480.15190627959408334No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8930.14309315155856164No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT8410.13476073959770474No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCACTCG8330.13347883006526523No Hit
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT7620.12210188296486446No Hit
GGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT7550.12098021212397989No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT6500.10415514951071114No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT6360.10191180782894199No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG6350.10175156913738706No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG6250.10014918222183765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39900.087.859651
AAGGGAC4500.085.641674
AACGGGT1050.085.044873
GTTACGG500.084.699061
TAAGCGG900.083.653391
CGCTACG3950.082.098613
AGGGAAT13700.081.646995
TAGGGCG750.081.464034
TCCGCTA4000.081.169931
CGGGTAG353.862624E-880.568825
GCTACGA4050.080.071474
AATGCGG950.079.250571
ATTACGG600.078.425051
AATACGG301.4452653E-678.425051
CGAGGGA3550.078.1101463
CGGGTAT850.077.4092565
CTACGAC4200.077.2117845
ACTAGGG3300.076.918932
AGGGCAA4950.076.90665
GGGAATG18300.076.533046
Produced by FastQC (version 0.11.5)