FastQCFastQC Report
Mon 27 Feb 2023
SRR3128494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823830
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158050.8665829600346524No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC46350.2541355279823229No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG42000.23028462082540588No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG41420.22710449987115028No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38790.21268429623374988No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACTTTGCG38200.20944934560786915No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22910.1256147776930964No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22090.12111874461983847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58000.083.203991
CGCTACG8250.075.7664643
CGTAGGG3600.075.739762
CTACGAC8350.075.4219365
AGGGATG30050.075.384485
GCTACGA8600.073.229444
AGGGAAT25050.072.79535
CGAAGGG18200.071.0329362
TAGCGGG8300.070.799532
GAGGGAT28700.070.742634
ACGACCA8750.070.362727
AGGGATC16800.070.217255
AGTAGGG27250.069.696882
AGGGTAC9300.069.233615
ACGGGAT7900.069.009724
GTACGGG2800.068.837372
AGAGGGC22600.068.833353
ATAGCGG3050.067.854341
TAGGGCG3050.067.80044
TAAGCGG5150.067.584841