FastQCFastQC Report
Mon 27 Feb 2023
SRR3128493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743275
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147420.8456497110323958No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC51080.2930117164532274No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACTTTGCG49820.28578394114525824No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG48170.27631899728958426No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG47240.27098421075274987No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37060.21258837532804634No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26480.1518980080595431No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20510.11765212029083191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53050.084.828011
AGGGATG29200.075.1661765
AGGGAAT24050.075.042035
ATAGCGG2450.074.8533551
TAAGGGA17600.073.696613
CGTAGGG3700.073.667742
CTACGAC7450.073.179635
GTAGGGC6000.072.8421553
GTAGGGA14200.071.1543053
ACGGGAT6700.070.8451464
GGTAAGG9600.070.534891
CCGTACA21050.069.6552053
ATAGGGA18150.069.6508943
TCCGCTA8000.069.3593141
AAGGGAT34400.069.128264
TAGCGGG8100.069.041942
ATAACGG1500.068.9674451
AGTAGGG25300.068.7277762
CGGGTAT1300.068.690825
TCCGTAC22500.068.299312