FastQCFastQC Report
Mon 27 Feb 2023
SRR3128490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132608
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477662.2397927795450454No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT59480.2789073284916872No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT57350.26891955764960085No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG52260.24505206770301904No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT49240.23089100294099996No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC42990.2015841636156293No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC27560.1292314386891543No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23950.11230380829482024No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC22690.10639554948682552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150000.087.742131
ATAGGGC13250.073.416953
AGGGATG31500.071.911565
ATAGGGA15450.071.1752853
TAGGGCA10200.070.4909064
AGATAGG12000.070.14041
AGTAGGG29350.069.330072
TAGGGAT11300.068.203594
GTAGGGC10950.067.808613
GATAGGG28700.066.806722
GAATAGG12100.065.674661
ATAAGGG24850.065.0539862
TAGAGGG35600.064.946682
GAGGGAT24800.064.806164
TATAGGG15600.064.767292
TCCGTAC6900.064.70182
CCGTACA6850.064.488033
GTAGGGT6200.064.427183
AAGGGAT30850.063.9787834
AATAGGG28800.063.9639742