FastQCFastQC Report
Mon 27 Feb 2023
SRR3128488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765394
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299691.0837153765430894No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC31340.11332923988408163TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG29640.10718183376401337TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27970.1011429112813581TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98200.083.1820451
ATAGGGC15400.073.852883
CGTAGGG9350.072.903272
ATAGCGG6050.072.330081
TAGGGCA13050.071.666374
ATAGGGA28350.071.6149143
ACGGGTA3050.070.881154
AGGGATG44450.070.416635
GAATAGG17050.070.373181
AGTAGGG46000.068.879922
ATAAGGG37400.068.629632
TATAGGG21250.068.579352
AGGGAAT31650.067.563245
GTAGGGC12400.067.084843
GGTAAGG14900.066.948521
AATAGGG41550.065.847992
TAGGGTA10350.065.841614
AGGGTAT14500.065.79625
TAGGGAT19500.065.555194
AGGGCAT24800.065.379295
Produced by FastQC (version 0.11.5)