FastQCFastQC Report
Mon 27 Feb 2023
SRR3128487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602688
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278361.0695096761501954No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC34910.13413056040524257TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG33810.12990416062163423TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG33770.12975047335677575TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91550.083.822521
CGTAGGG6600.079.0395052
ATAACGG2400.078.3696061
ACGGGAT9300.074.286164
GTAGGGC12450.073.231443
TAGGGCA11150.072.0766754
AAGGGTA14650.071.217864
AGGGATG39850.071.116585
GAATAGG16600.070.532641
ATAGGGA27750.070.113653
AGTACGG4200.069.413071
GTAGGGA21300.069.281173
CCGTACA9150.068.8254243
ATAGGGC14750.068.184713
ATAAGGG31300.067.8670962
TAGGGTC4850.067.831254
ACGGGTA2150.067.763654
CGAAGGG27100.067.459872
AGTAGGG40850.067.4172062
TAGAGGG44750.066.267642
Produced by FastQC (version 0.11.5)