FastQCFastQC Report
Mon 27 Feb 2023
SRR3128486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914293
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342121.1739382416249842No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC39560.13574475867731897TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG38280.1313526127949386TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG34480.11831342970662181TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29320.10060759161827584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105950.083.763131
CGTAGGG7800.079.549352
ATAGGGA28750.075.8499763
ATAGGGC17950.072.787313
GAATAGG18550.071.788471
TAGGGCA14050.070.9144744
AGGGATG44500.070.443545
ACGGGTA3650.069.530644
GAGGGAT38600.068.670014
AGTAGGG50750.068.6340262
ACGGGAT10500.068.482174
CGAAGGG28600.068.044282
TAGAGGG48700.067.662242
ACTAGGG14800.067.651152
AAGGGAT48050.066.999654
AGAGGGC41350.066.9445343
TATAGGG21850.066.906192
GTAGGGA24700.066.595733
TAAGGGA24850.066.382883
AGTAAGG20350.066.3635561
Produced by FastQC (version 0.11.5)