FastQCFastQC Report
Mon 27 Feb 2023
SRR3128485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887087
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90241.017262117469876No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60590.6830220711159108No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT34860.3929716025598391No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG34600.39004066117528496No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC30580.34472379822948596Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG28160.31744349765017416No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17690.1994167426644737No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAA12810.14440522744668785No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATG12070.13606331735218755No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT11160.12580502250624798No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT11110.12524137993229525No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10720.12084496785546402No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG9420.10619026093269318No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9250.10427387618125393No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT9110.1026956769741863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36050.087.043561
CGCTACG11100.085.1036763
GCTACGA11350.083.643224
ATAGCGG1700.083.021081
AGGGATG20100.083.005625
AGGGTAT4250.082.936855
TCCGCTA11550.082.6856461
CGTAGGG1650.082.648342
GGGCGAT3450.081.734866
GCGGGAT4150.081.537914
AGGGCTA4150.081.537915
CTACGAC11800.080.851725
AGGGAAT18800.078.995875
CGACCAA11800.078.86038
GACCGTA301.4563175E-678.329258
AAGGGAT20200.078.174144
CGAAGGG5850.077.971512
ACGACCA12000.077.93767
GATGACC9950.077.935638
AAGGGTA6350.077.712484
Produced by FastQC (version 0.11.5)