FastQCFastQC Report
Mon 27 Feb 2023
SRR3128484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842550
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82360.9775087531897217No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54880.651356002611121No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT44860.532431309714557No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG39000.46288054121417127No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC34570.41030205922497176Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG32880.3902439024390244No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA21600.25636460744169487No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATG15680.18610171503174885No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT12500.1483591478250549No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAA12210.14491721559551363No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT11700.1388641623642514No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG11250.1335232330425494No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10150.12046762803394456No Hit
TAGACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT10000.1186873182600439No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT9990.11856863094178385No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCAAACAGTTC9650.11453326212094238No Hit
GAACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT9280.11014183134532074No Hit
AGAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT9030.10717464838881965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT353.601599E-1093.989853
CGTAGGG1750.088.6189962
CGTTTTT36100.086.2046361
ATTGCGG600.086.182931
TACGGGT401.036824E-982.241133
CGCTACG10350.082.1843643
AAGGGTA6350.082.1486054
ACGGGTA750.081.457874
TCCGCTA10700.081.277021
AGGGATG21700.081.212435
GCTACGA10500.081.010314
CGAGGGA5800.080.215483
AGGGAAT16400.079.9486855
CGAAGGG5650.079.8497852
GGGAATG21150.079.102576
AGGGTAT4100.079.089025
CGGGAAT3100.078.83025
CTACGAC10800.078.760015
GTTACGG600.078.348121
CTAGCGG600.078.348121
Produced by FastQC (version 0.11.5)