FastQCFastQC Report
Mon 27 Feb 2023
SRR3128483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934783
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104681.1198320893726137No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66660.7131066782344138No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG44400.4749765453586554No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC40080.4287626112156512Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG36290.3882184421411172No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT23060.24668826882816652No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT14230.15222784325346098No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAA13810.14773482187844666No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT12640.13521854804804967No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12600.13479064125042925No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11830.12655343539623637No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT11450.12248832081884246No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG10620.11360925476821893No Hit
TAGACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT10380.11104181398249648No Hit
GAACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT10380.11104181398249648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT500.093.996213
GGGTACG1200.090.07976
CGTTTTT39900.086.876091
ACGGGTA1150.085.8226244
CGTAGGG2250.085.650152
CGGGATT2000.084.596595
GGGCGAT3250.083.8735356
GTTACGG452.7284841E-1183.61481
AATGCGG1300.083.21281
CGCTACG11750.083.196643
GCGGGAT5100.082.937824
TCCGCTA12000.081.524421
TTACGGG1500.081.4720842
AAGGGTA6950.081.1478044
AGGGTAC4350.081.031215
CGAGGGA5300.080.694853
GCACGGG2100.080.57682
GCGATAA700.080.568178
GGATTCG700.080.568177
GCTACGA12100.080.401714
Produced by FastQC (version 0.11.5)