FastQCFastQC Report
Mon 27 Feb 2023
SRR3128482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259306
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA40251.552220156880288No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.8796556963587422No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG17600.6787347766731198No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16510.6366994978905232No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC14840.5722968230584715No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG12530.4832128836201245No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG9890.38140266711915655No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAACGGAATC9300.35864962631022806No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT6960.268408752593461No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6420.24758393558189937No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6270.2417992641897989No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6020.23215814520296482No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5270.20323478824246258No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG5030.19397931401510185No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT5000.19282237973668176No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG4810.18549512930668785No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC4560.17585401031985376No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC3790.14615936384040476No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG3670.14153162672672442No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG3120.12032116495568942No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG3030.11685036212042915No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC2870.11068004596885533No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2720.10489537457675487No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT2710.1045097298172815No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2660.10258150601991468No Hit
GGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT2650.10219586126044133No Hit
ATCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC2640.10181021650096797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG156.875294E-494.0715941
GCTAAGG156.875294E-494.0715941
ATACCGG156.875294E-494.0715941
GGATTCG254.9106893E-793.999047
ATTCGAT156.896417E-493.999039
CGAGAAT156.896417E-493.999033
TGTACGT201.8352956E-593.999037
CGTTTTT20000.087.486591
CGTAGGG550.085.470152
AGGGCGA500.084.599145
ATAGCGG452.7284841E-1183.6191941
CACCGGG452.7284841E-1183.5708162
GGTATAT452.7284841E-1183.55477
AATGCGG401.02591E-982.312641
GGATAGC800.082.249157
CGAGGGA1200.082.249153
TAGGGCG401.031367E-982.249154
TAGCGGG750.081.4815442
TATAGGG1000.079.914592
GGTAAGG950.079.218181
Produced by FastQC (version 0.11.5)