FastQCFastQC Report
Mon 27 Feb 2023
SRR3128481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258408
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA54012.090105569487013No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.8420791925946565No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG20240.7832574842884122No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20200.7817095445961425No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC17250.6675489922912604No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG17210.6660010525989907No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAACGGAATC11730.45393331475805704No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG11180.43264914398935017No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT7340.2840469335314696No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT6810.2635367326088976No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6210.2403176372248537No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG6200.23993065230178634No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC6170.23876969753258412No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6150.23799572768644933No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAATCG5690.22019442122534907No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5300.20510200922572056No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5270.2039410544565184No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG3720.1439583913810718No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG3650.1412494969196No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC3600.13931457230426303No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT3590.13892758738119562No Hit
ATCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC3590.13892758738119562No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG3590.13892758738119562No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT3480.13467075322745425No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3360.1300269341506455No Hit
GATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC3030.11725643168942139No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT2860.11067768799727562No Hit
GACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC2710.10487291415126467No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2700.10448592922819727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT301.322951E-893.9883964
GGGCTAA156.8994943E-493.9883966
TACGGGT201.8363147E-593.9883963
CCGTAAA156.8994943E-493.9883963
CGGGATA201.8363147E-593.9883965
CGTAAAC156.8994943E-493.9883964
GTACGGG500.093.9883962
GACTAGA156.8994943E-493.9883969
ATGGGCG353.583409E-1093.988394
ATAGCGG353.583409E-1093.988391
TAACGGG550.093.988392
CGTAGGG353.583409E-1093.988392
TAAGGGT353.583409E-1093.988393
CGTTTTT17550.087.294061
GGGTACG600.086.156036
AATAGGG2850.085.743792
ATAGGGC1500.084.5895543
ACTAGGG1000.084.5895542
TACGGGA452.7284841E-1183.545243
TATAGGG900.083.545242
Produced by FastQC (version 0.11.5)